imagequant analysis program Search Results


phorphorimager analysis program imagequant  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc phorphorimager analysis program imagequant
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Phorphorimager Analysis Program Imagequant, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phorphorimager analysis program imagequant/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
phorphorimager analysis program imagequant - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
gel analysis program imagequant  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc gel analysis program imagequant
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Gel Analysis Program Imagequant, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gel analysis program imagequant/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
gel analysis program imagequant - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
imagequant analysis program  (FUJIFILM)
90
FUJIFILM imagequant analysis program
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Analysis Program, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant analysis program/product/FUJIFILM
Average 90 stars, based on 1 article reviews
imagequant analysis program - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
analysis program imagequant 3.3  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc analysis program imagequant 3.3
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Analysis Program Imagequant 3.3, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/analysis program imagequant 3.3/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
analysis program imagequant 3.3 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
imagequant radioactivity analysis program  (Molecular Dynamics Inc)
90
Molecular Dynamics Inc imagequant radioactivity analysis program
Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program <t>ImageQuant.</t> Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.
Imagequant Radioactivity Analysis Program, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imagequant radioactivity analysis program/product/Molecular Dynamics Inc
Average 90 stars, based on 1 article reviews
imagequant radioactivity analysis program - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program ImageQuant. Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.

Journal:

Article Title: Evidence for κ - and μ -Opioid Receptor Expression in C6 Glioma Cells

doi:

Figure Lengend Snippet: Semiquantification of opioid receptor mRNA expression after DMI treatment. Expression levels (at a number of thermocycles determined to fall within the linear range for both GAPDH and opioid receptor cDNA amplification) are shown as a function of time of DMI treatment. Product (5 μl) from each PCR reaction (RTs from C6 and DMI-treated C6) was taken over a range of 15-35 thermocycles and analyzed via agarose electrophoresis, followed by Southern blotting using radiolabeled probes specific for GAPDH, and the μ- or κ-opioid receptor cDNA sequence amplified. Each blot was then analyzed with the phosphorimaging program ImageQuant. Normalization of opioid receptor expression is given as the density of the receptor band divided by the density of the GAPDH band. A: Normalized κ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear part of the amplification curve. B: Normalized μ expression after 0, 0.5, 1, 1.5, 2, and 20 h of DMI treatment. Shown is the comparison at 24 cycles, a point within the linear amplification curve. This experiment was repeated in triplicate, using different preparations of control and treated C6 cells. Data represent the average ± SEM values of three experiments.

Article Snippet: Band densities were quantified with the phorphorimager analysis program ImageQuant by Molecular Dynamics.

Techniques: Expressing, Amplification, Electrophoresis, Southern Blot, Sequencing, Comparison, Control